Jackie Mittoo (3 March 1948 — 16 December 1990) was a Jamaican keyboardist, songwriter and musical director. He was a founding member of The Skatalites and was a mentor to many younger performers, primarily through his work as musical director for the Studio One record label.

He was born Donat Roy Mittoo in Browns Town, Saint Ann Parish, Jamaica, and began learning to play the piano when he was four under the tutelage of his grandmother.

In the 1960s he was a member of The Skatalites, The Rivals, The Sheiks, The Soul Brothers and The Soul Vendors. Among Mittoo's contributions in the mid to late 1960s were "Darker Shade of Black" (the basis for Frankie Paul's "Pass the Tu Sheng Peng"), Freddie McGregor's "Bobby Babylon", Alton Ellis' "I'm Still in Love with You", The Cables' rocksteady anthem "Baby Why" and Marcia Griffiths' first hit, "Feel Like Jumping". He played for Lloyd "Matador" Daley in 1968 and 1969.